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Phis2 酵母单杂

http://www.bioon.com.cn/doc/showarticle.asp?newsid=101857 Webb质粒名称：pHis2 出品公司：Clontech 质粒类型： 酵母单杂交系统 克隆方法： 多克隆位点，限制性内切酶 载体大小： 7207bp 原核抗性： Kanamycin (卡那霉素) 真核抗性：TRP1 (HIS3 selection) 备注： Clone in cis-acting sequences for use with 1-hybrid system 载体介绍 pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify …

pHis2载体质粒图谱、序列、价格、抗性、大小等基本信息_生物风 …

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pHIS2酵母单杂交质粒-北京庄盟国际生物基因科技有限公司

http://www.biofeng.com/zaiti/jiaomuzajiao/pHis2.html Webb1 juni 2024 · Clone the products of the PCR reactions carried out in step 3 into the pHIS2 T-vector obtained in step 5. The T-A ligation system includes 0.2 μg of T-vector of pHIS2, 0.5 μL of PCR products (Y1 + Y3 and Y2 + Y3), 1 μL of 10× ligation buffer, 3 U of T4 ligase, and PEG6000 at final concentration of 10% (w/v). Webb22 juli 2013 · 酵母单杂交系统 Clontech pHis2说明书 Description pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for use with the BD Matchmaker One-Hybrid Library Construction & Screening Kit （#K1617-1）. how to get static out of dry hair

Phis2 TaKaRa Bioz

Category:pHIS2酵母单杂交质粒_价格-品牌-详情介绍_丁香通

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Phis2 酵母单杂

人机验证 - scimall.org.cn

Webb1、文库质量评估：理论+涂板评价 交付材料：反馈质量评估结果 2、载体构建：基因合成诱饵基因 构建Phis2载体 交付材料：含诱饵基因的质粒 3、启动子背景筛查：Binding seq基因启动子的背景筛查 交付材料：阳性克隆测序结果 word项目报告 4、文库筛选：诱饵质粒转化和文库质粒转化 酵母筛选和3AT条件优化 筛选结果测序 交付材料：阳性克隆测序结果 … Webb丁香通为您提供phis2酵母单杂交质粒商品详情介绍：价格：¥1000，货号：qc0421，品牌：钦诚生物，产地：上海，供应商：钦诚生物，保质期：2年，详见丁香通phis2酵母单杂交质粒商品详情页；

Phis2 酵母单杂

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Webb人机验证 - scimall.org.cn Webb22 juli 2013 · 酵母单杂交系统 Clontech pHis2说明书 . Description. pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA …

Webb1 apr. 2024 · The Y187 strain was prepared for the Y1H assay, we mixed pAD-CmBBX7 and pAD-GUS with pHIS2-CmFTL1pro respectively, and transformed them into Y187 strain using the lithium acetate method.Then inoculated on SD/His-Trp-Leu-plates, and screened for the lowest concentration range of 3-AT that was sufficient to inhibit the growth of … http://www.honorgene.com/product_list/carrier_library/123112.html

Webb1 apr. 2016 · 豆丁网是面向全球的中文社会化阅读分享平台，拥有商业,教育,研究报告,行业资料,学术论文,认证考试,星座 ... WebbpHis2 价格： 1000元 联系方式： I47-825O-882O 立刻购买 买家导航 载体基本信息 载体质粒图谱和多克隆位点信息 载体简介 载体序列 LOCUS pHIS-2 7207 bp DNA SYN DEFINITION pHIS-2 ACCESSION KEYWORDS SOURCE ORGANISM …

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Webb1、pHis2-Bait或pAbAi-Bait和AD-prey质粒 2、或者Bait和Prey序列，质粒由我们构建 1、提供详细的、完整的实验报告，清晰的实验图片 2、在受体菌的阳性菌 自激活和毒性验证: … johnny winter rock and roll medleyWebbpHIS2 PVT4037 2ug Lot no 1901093 Store at -20℃ Expiry date June.2024 pHIS2 Information Promoters: TRP1, T7, T3 Replicon: pUC ori, FI ori Plasmid classification: yeast series, yeast one hybrid vector Plasmid size: 7207bp … how to get statistics in excelhttp://www.bioon.com.cn/doc/showarticle.asp?newsid=96627 johnny winter rock and roll hoochie koo liveWebb1 mars 2024 · MdGSTF6-p-pHIS2 vectors were first grown on −Trp/−His medium containing 3-amino-1,2,4-triazole (3-AT) for screening. The concentration of 3-AT used was 100 mM. how to get static refinerWebb实验原理: 真核生物基因的转录起始需转录因子参与，转录因子通常由DNA结合结构域 (ONA- binding domain, BD)和转录激活结构域 (acivationdomain, AD)组成.用于酵母单杂交系统的 … johnny winter rock and roll hoochie kooWebb质粒名称：pHis2 出品公司：Clontech 质粒类型： 酵母单杂交系统 克隆方法： 多克隆位点，限制性内切酶 载体大小： 7207bp 原核抗性： Kanamycin (卡那霉素) 真核抗 … how to get static out of polyesterWebb酵母单杂交体系是在酵母双杂交体系基本原理的基础上，通过观察酵母细胞内报告基因的表达状况，在酵母细胞内研究DNA与蛋白质之间的相互作用的一种技术。 许多真核生物的 … how to get static pressure