WebTen micrograms of total RNA, prepared as described above, was resolved on 6.3% formaldehyde gels containing 1% agarose and blotted overnight to a Hybond-XL … Web11 jul. 2024 · MDA-MB-231 was purchased from Korean Cell Line Bank and maintained at 1 × 10 6 cells/mL in RPMI1640 supplemented with 300 mg/L L-glutamine, 25 mM HEPES, 25 mM NaHCO 3, 10% fetal bovine serum, 100 IU/mL penicillin G, and 100 μg/mL streptomycin at 37 °C in a humidified atmosphere containing 5% CO 2 and 95% ambient air.

Sensors Free Full-Text The Effects of Dithiothreitol on DNA

WebTen micrograms of total RNA, prepared as described above, was resolved on 6.3% formaldehyde gels containing 1% agarose and blotted overnight to a Hybond-XL membrane (Amersham Biosciences). DNA probes, complementary to centromeric dg-dh repeats and actin, were generated with Random Primed DNA Labeling Kit (Roche). Web26 feb. 2013 · We have used Hybond-N, Hybond-N + and Hybond-XL membranes (pore size of 0.45 μm) with equal results, however other types of membranes are likely to … substitutes for bean bag chair

A 7-Step Guide to Western Blotting Cytiva

WebHybond®-XL hybridization membranes Product Comparison Guide Use the product attributes below to configure the comparison table. (Select up to 3 total.) Select Attribute … WebIs it possible to use a normal blotting apparatus like the ones normally used for proteins? We normally use either the Hybond-N+ or the Hybond-XL membranes. Are they good for … WebAmersham Biosciences Scanners - GE Healthcare Life Sciences paint colors in rooms