WebThe enzyme exhibited the highest specific activity of 16.0 U/mg towards colloidal chitin, followed by ethylene glycol chitin (5.4 U/mg) and ball milled chitin (0.4 U/mg). The Km and Vmax values of ...
Thermogel - an overview ScienceDirect Topics
WebMar 10, 2014 · For example, assessment of chitinase activity can be accomplished with solubilized substrates such as ethylene glycol chitin, carboxymethyl chitin and 6-O-hydroxypropyl-chitin, or insoluble modified chitin substrates such as chitin azure and tritium-labeled chitin [2, 5]. However, the use of native unmodified substrates is highly … WebFeb 15, 2013 · The chemical structure of glycol chitosan and glycol chitin was also confirmed by FT-IR spectroscopy, as presented in Fig. 1 b. The broad band at 3400 cm … bakery jobs hiring near me
TLC analysis of the hydrolysis products of colloidal chitin (a ...
WebEthylene glycol chitosan (EGC, 40 mg) was treated at 4 ºC in a mixture containing 400 mg of NaHCO3 and 4.5 ml of acetic anhydride and kept at 4 ºC for 24 h. After addition of 200 … The first step involved the synthesis of 1-butyl-3-methylimidazolium chloride. In a 250 ml round-bottomed flask equipped with a Teflon-coated magnetic stirring bar, 1-methylimidazole (0.10 mol) was reacted with 1-chlorobutane (0.11 mol) added at once to 100 ml of acetonitrile, which was utilized as a reaction … See more Chitin dissolution was carried out using the EasyMax 102 system (Mettler Toledo, Switzerland), which allows the high-precision control of a broad range of reaction parameters such as temperature, pH, stirring speed, … See more The nanomechanical properties of the chitin–EG membrane were determined with the use of atomic force microscopy (AFM). This … See more A MIRA3 scanning electron microscope (SEM) (Tescan, Czech Republic) was used to assess the surface. This was done using an acceleration voltage of 2 kV and a working distance of 2 mm. A thin Au–Pd layer with a thickness … See more The wettability and surface free energy of the prepared chitin–EG thin films were determined by static contact angle measurements, with distilled water and diiodomethane as the polar and dispersion components, … See more WebFeb 10, 2024 · Here Of Chi-h, a Chi-h from Ostrinia furnacalis, was investigated. Crystal structures of both Of Chi-h and its complex with chitoheptaose ( (GlcN) 7) reveal that Of Chi-h possesses a long and asymmetric substrate binding cleft, which is a typical characteristics of a processive exo-chitinase. The structural comparison between Of Chi-h and its ... bakery jobs atlanta