Chloramphenicol selection marker
WebChloramphenicol is a synthetic antibiotic, isolated from strains of Streptomyces venezuelae. It is often used for bacterial selection in molecular biology applications at 10-20μg/mL … WebSelection markers are protein coding sequences that confer a selective advantage or disadvantage to host chassis. For example, a common type of prokaryotic selection marker is one that confers resistance to a particular antibiotic. ... chloramphenicol acetyltransferase (forwards, CmF) [cf. BBa_J31004] P62577: none: 660: In stock: …
Chloramphenicol selection marker
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WebChloramphenicol shows a potent but delayed parasiticidal effect, allowing the use of E. coli chloramphenicol acetyl transferase (CAT) not only as reporter enzyme but also as a … WebNov 16, 2013 · We routinely use the chloramphenicol InTag adaptor for IgG reformatting of ‘Fab-on-phage’ clones, although InTag adaptors with a different selection marker (e.g. zeocin) can also be used. We also use InTag positive selection cloning for further optimization of lead IgG antibody candidates using heavy or light chain shuffling …
WebUsing Ampicillin as a Selection Marker for Plasmid DNA Isolation. There are several selectable markers that can be used to identify bacterial cells that contain a specific trait. … WebA comparison of chloramphenicol selection (7.5 μg/ml) is shown in (B), with pIMC exhibiting uniform colony size compared to pPL2 transformed EGDe. ... 12–13% of the randomly selected bacterial ...
WebMay 13, 2024 · One colony each of DH1PEPA (pBRT1Nc), DH1 (pBRT1Nc) or WT05 (pBRT1Nc) were picked from chloramphenicol selective plates and reinoculated into 5 ml of LB or LB–aro mix without antibiotic selection and cultured for approximately 16 h … Web301 Moved Permanently. nginx
WebMar 14, 2024 · Therefore, we have sought to develop a second transformant selection marker. C. merolae cells are sensitive to chloramphenicol (CP), an inhibitor of protein synthesis in bacteria, mitochondria and chloroplasts, which has been widely used for the selection of transformants in other organisms ( Minoda et al., 2004 ).
WebTemplate plasmid which encodes chloramphenicol resistance gene for positive selection and a toxin gene (relE) under the control of rhamnose induceable promoter (PrhaB) for … lko mahotsavWebAug 16, 2024 · Conclusion: Chloramphenicol was proved to be an ideal selection marker for A. caldus. Novel plasmids carrying cat gene were constructed. The utilization of these vectors will undoubtedly facilitate efficient genetic manipulations and accelerate the research progress in A. caldus. MeSH terms Acidithiobacillus / drug effects can tesla autopilot honkWebNov 5, 1993 · Introduction of the chloramphenicol acetyltransferase (CAT) gene fused to Toxoplasma flanking sequences followed by chloramphenicol selection resulted in … l kostenWebChloramphenicol resistance conferred by chloramphenicol acetyltransferase (cat) can be used for a section of unicellular cyanobacteria and Anabaena sp. strain 90 [46,47]. Other antibiotics, such as tetracycline and rifampicin, are light sensitive and are of little use for cyanobacterial selection [42,48]. lko to jammu trainWebChloramphenicol is an antibiotic useful for the treatment of a number of bacterial infections. [3] This includes use as an eye ointment to treat conjunctivitis. [4] By mouth or by … lkotonWebChloramphenicol inhibits protein synthesis by binding to the 50S ribosomal subunit and inhibiting peptide chain elongation. 99 The major chloramphenicol resistance mechanism … cantina kuka coiteWeb3 Chloramphenicol selection marker as content of the GATEWAY-gene cassette. 4 Size of BiFC-expression construct includes: fluorophore fragment [N-terminal fragment (aa 1–173) and C-terminal fragment (aa 156–239)], Linker-Tag, … lkotx